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Plant RNA Regulation
9/1/2016 by  
Plant RNA Regulation
The Plant RNA Regulation (PRR) experiment builds on the previous flight results from the Plant Signaling experiment performed in 2011. Plant Signaling focused on responses in an important second messenger in plants; the inositol (1,4,5) triphosphate pathway. The following PRR experiment aims to study the plant adaption and responses to space environments through the novel regulation of small RNAs and proteins in roots and shoots. The long term goal is to understand the molecular background and mechanisms by which plants sense and adapt to changes in their environment and how they regulate these responses. This knowledge will be important for designing plants able to withstand adverse conditions in space as well as on ground. The experiment was launched 18th of July 2016 in the Space-X 9 rocket and the experiment runs will be executed from the 22nd of August until 7th of September.
The experiment will take place inside Experiment Containers in the EMCS on the International Space station. Astronauts will place the Experiment Containers with Arabidopsis thaliana seeds, inside the EMCS. One group of seeds will be exposed to µg and the control group to 1 g. The experiment will be started by hydrating the seeds and let them grow for 4 and 6 days respectively in optimal environmental conditions with regard to temperature, light regime and air flow. At experiment end, the samples will be removed from the EMCS and put into a freezer at -80 degrees. Later they will be returned to ground for further proteomic and transcriptional analyses at PIs laboratory.
Tropi Seed Cassettes were used for Plant RNA Regulation
Experiment name: Plant RNA regulation
Facility: EMCS
Test subject: Arabidopsis thaliana
Experiment duration: 2 runs x 4 and 6 days
Growth substrate: filter paper
Water source: internal EUE water
Principle Investigator: Imara Perera
Payload Developer: NASA Ames Research  Center
Launch vehicle: Space-X 9
Performance: August/September 2016
Point of contact:
Principal Investigator: Dr. Imara Perera, North Carolina State University,